An unexpected gene cluster for downstream degradation of alkylphenols in Sphingomonas sp strain TTNP3

作者：Kolvenbach Boris A^*; Dobrowinski Hyazinth; Fousek Jan; Vlcek Cestmir; Schaeffer Andreas; Gabriel Frederic L P; Kohler Hans Peter E; Corvini Philippe F X

来源：Applied Microbiology and Biotechnology, 2012, 93(3): 1315-1324.

DOI：10.1007/s00253-011-3451-8

• Hydroquinone
• Degradation
• Sphingomonas
• Nonylphenol
• Bisphenol A

In silico analysis of nucleotide sequences flanking the recently found hydroquinone dioxygenase in Sphingomonas sp. strain TTNP3 revealed a gene cluster that encodes a hydroquinone catabolic pathway. In addition to the two open-reading frames encoding the recently characterized hydroquinone dioxygenase, the cluster consisted of six open-reading frames. We were able to express the three open-reading frames, hqdC, hqdD, and hqdE, and demonstrated that the three gene products, HqdC, HqdD, and HqdE had 4-hydroxymuconic semialdehyde dehydrogenase, maleylacetate reductase, and intradiol dioxygenase activity, respectively. Surprisingly, the gene cluster showed similarities to functionally related clusters found in members of the alpha- and gamma-proteobacteria rather than to those found in other members of the genus Sphingomonas sensu latu.

• 出版日期2012-2
• 单位南京大学