HOMING OF A DNA ENDONUCLEASE GENE BY MEIOTIC GENE CONVERSION IN SACCHAROMYCES-CEREVISIAE

作者：GIMBLE FS; THORNER J

来源：Nature, 1992, 357(6376): 301-306.

DOI：10.1038/357301a0

An unusual protein splicing reaction joins the N-terminal segment (A) and the C-terminal segment (C) of the 119K primary translation product (ABC) of the yeast VMA1 gene to yield a 69K vacuolar H+-ATPase subunit (AC) and an internal 50K polypeptide (B). This 50K protein is a site-specific DNA endonuclease that shares 34% identity with the homothallic switching endonuclease. The site cleaved by the VMA1-derived endonuclease exists in a VMA1 allele that lacks the derived endonuclease segment of the open reading frame. Cleavage at this site only occurs during meiosis and initiates 'homing', a genetic event that converts a VMA1 allele lacking the endonuclease coding sequence into one that contains it.

• 出版日期1992-5-28