A new effective process for production of curdlan oligosaccharides based on alkali-neutralization treatment and acid hydrolysis of curdlan particles in water suspension

作者：Li Jing; Zhu Li; Zheng Zhi Yong; Zhan Xiao Bei; Lin Chi Chung^*; Zong Yu; Li Wei Jiang

来源：Applied Microbiology and Biotechnology, 2013, 97(19): 8495-8503.

DOI：10.1007/s00253-013-5125-1

• Curdlan
• beta-1,3-oligosaccharides
• Alkali-neutralization treatment
• Double-layer structure model
• Acid hydrolysis

Biologically active beta-1,3-oligosaccharides with rapidly growing biomedical applications are produced from hydrolysis of curdlan polysaccharide. The water-insoluble curdlan impedes its hydrolysis efficiency which is enhanced by our newly developed alkali-neutralization treatment process to increase the stability of curdlan suspension to more than 20 days, while the untreated control settled within 5 min. A putative double-layer structure model comprising of a compact core and a hydrated outer layer was proposed to describe the treated curdlan particles based on sedimentation and scanning electron microscopy observation. This model was verified by single- and two-step acid hydrolysis, indicative of the reduced susceptibility to hydrolysis when close to the compact core. Electrospray ionization-mass spectrometry, thin-layer chromatography analyses, and effective HPLC procedure led to the development of improved process to produce purified individual beta-1,3-oligosaccharides with degrees of polymerization from 2 to 10 and potential for biomedical applications from curdlan hydrolyzate. Our new curdlan oligosaccharide production process offers an even better alternative to the previously published processes.

• 出版日期2013-10
• 单位江南大学