A high performance liquid chromatography method for simultaneous determination of rosiglitazone and gemfibrozil in human plasma

作者：Kang Xin; Wang Feng; Xie Zhihong; Li Huande^*

来源：Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2009, 877(7): 645-648.

DOI：10.1016/j.jchromb.2009.01.001

• Rosiglitazone
• Gemfibrozil
• HPLC

A high performance liquid chromatography (HPLC) method was developed for the simultaneous determination of rosiglitazone and gemfibrozil in human plasma using alpha-asarone as an internal standard (IS). Plasma samples were pretreated by protein precipitation. The analytes were separated on a Macherey-Nagel Nucleodur C(18) (250 min x 4,6 mm, 5 mu m) column using acetonitrile and 30 mmol/l ammonium acetate solution (including 0.1% methanoic acid) as mobile phase which was delivered at 1 : 2 ml/min. A gradient elution program was adopted to adjust proportion of solvent in mobile phase. A time program was used to regulate conditions of fluorescence detection. The method was validated over the concentration range of 5.0-751.3 ng/ml for rosiglitazone and 0.5-75.4 mu g/ml for gemfibrozil with acceptable accuracy, precision and extraction recoveries. This method is suitable for routine therapeutic drug monitoring and pharmacokinetic interaction study of the two drugs.

• 出版日期2009-3-1
• 单位中南大学