Dual-wavelength photothermal optical coherence tomography for imaging microvasculature blood oxygen saturation

作者：Yin Biwei; Kuranov Roman V^*; McElroy Austin B; Kazmi Shams; Dunn Andrew K; Duong Timothy Q; Milner Thomas E

来源：Journal of Biomedical Optics, 2013, 18(5): 056005.

DOI：10.1117/1.JBO.18.5.056005

• optical coherence tomography
• dual-wavelength
• imaging
• phase-sensitive
• microvasculature
• hemoglobin oxygen saturation
• phantom

A swept-source dual-wavelength photothermal (DWP) optical coherence tomography (OCT) system is demonstrated for quantitative imaging of microvasculature oxygen saturation. DWP-OCT is capable of recording three-dimensional images of tissue and depth-resolved phase variation in response to photothermal excitation. A 1,064-nm OCT probe and 770-nm and 800-nm photothermal excitation beams are combined in a single-mode optical fiber to measure microvasculature hemoglobin oxygen saturation (SO2) levels in phantom blood vessels with a range of blood flow speeds (0 to 17 mm/s). A 50-mu m-diameter blood vessel phantom is imaged, and SO2 levels are measured using DWP-OCT and compared with values provided by a commercial oximeter at various blood oxygen concentrations. The influences of blood flow speed and mechanisms of SNR phase degradation on the accuracy of SO2 measurement are identified and investigated. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI. [DOI: 10.1117/1.JBO.18.5.056005]

• 出版日期2013-5