Ascorbic acid, alpha-tocopherol, and beta-carotene reduce oxidative stress and proinflammatory cytokines in mononuclear cells of Alzheimer%26apos;s disease patients

作者：de Oliveira Barbara Fonseca; Veloso Clara Araujo; Nogueira Machado Jose Augusto; de Moraes Edgar Nunes; dos Santos Rodrigo Ribeiro; Gualberto Cintra Marco Tulio; Chaves Miriam Martins^*

来源：Nutritional Neuroscience, 2012, 15(6): 244-251.

DOI：10.1179/1476830512Y.0000000019

• Alzheimer%26apos
• s disease
• Antioxidants
• Mononuclear cells
• Oxidativestress
• Vitamins

Objectives: The in vitro effect of a vitamin complex in generating and reducing oxidative species in peripheral blood mononuclear cells (PBMNC) and plasma of patients with Alzheimer%26apos;s disease (AD) and healthy subjects (HS) was evaluated. %26lt;br%26gt;Methods: Two concentrations of a vitamin complex ([A] and [20A]) with ascorbic acid, alpha-tocopherol, and beta-carotene were incubated with either mononuclear cells or plasma. The generation of oxidizing species was measured in a luminol-dependent chemiluminescence assay and the reducing response by the MTT dye reduction assay. The levels of cytokines (interleukin [IL]-1 beta, IL-6, and IL-4) were measured by sandwich enzyme-linked immunosorbent assay. %26lt;br%26gt;Results: Our results demonstrated that the increase in the vitamin complex concentration reduced the reactive oxygen species (ROS) production and enhanced cellular reduction capacity in cells of AD patients in concentration [20A]. Plasma reduction capacity rose significantly for both groups (AD and HS). Concentration [A] did not alter the IL-1 beta production, increased IL-4 production in both groups and lowered IL-6 production in AD cells. Concentration [20A] increased pro-inflammatory cytokines (IL-1 beta and IL-6) and decreased IL-4 production by PBMNC of HS leading to a pro-inflammatory status. %26lt;br%26gt;Discussion: The antioxidant vitamin complex was effective in reducing oxidative stress in PBMNC of AD patients by lowering ROS production, improving cellular antioxidant capacities and modifying cytokine induced inflammation.

• 出版日期2012-11