Allograft inflammatory factor-1 in disk abalone (Haliotis discus discus): Molecular cloning, transcriptional regulation against immune challenge and tissue injury

作者：De Zoysa Mahanama; Nikapitiya Chamilani; Kim Yucheol; Oh Chulhong; Kang Do Hyung; Whang Ilson; Kim Se Jae; Lee Jae Seong; Choi Cheol Young; Lee Jehee

来源：Fish & Shellfish Immunology, 2010, 29(2): 319-326.

DOI：10.1016/j.fsi.2010.04.006

• Abalone Haliotis discus discus
• Allograft inflammatory factor-1
• Invertebrate
• Hemocytes
• Tissue injury

Here, we report the identification and characterization of allograft inflammatory factor-1 (AIF-1) from disk abalone Haliotis discus discus that was denoted as AbAIF-1. The full-length cDNA of AbAIF-1 consists of a coding region (453 bp) for 151 amino acids with a 17 kDa molecular mass. Analysis of AbAIF-1 sequence showed that it shares characteristic two EF hand Ca+2-binding motifs. Results from phylogenetic analysis further confirm that AbAIF-1 is a member of the AIF-1 family similar to invertebrate and vertebrate counterparts suggesting it has high evolutional conservation.
Tissue-specific expression and transcriptional regulation of AbAIF-1 were analyzed after bacteria (Vibrio alginolyticus, Vibrio parahemolyticus and Lysteria monocytogenes), viral hemorrhagic septicemia virus (VHSV) immune challenge and during tissue injury by quantitative real-time PCR. It is shown that the expression of AbAIF-1 mRNA was expressed ubiquitously in all selected tissues in constitutive manner showing the highest level in hemocytes. Upon bacteria and VHSV challenge, AbAIF-1 showed the significant up-regulation in hemocytes than gills. After the tissue injury in shell and mantle, AbAIF-1 and antioxidant selenium-dependant glutathione peroxidase (SeGPx) transcripts were significantly upregulated in abalone hemocytes. Taken together, these findings suggest that AIF-1 could response against the pathogenic challenge or tissue injury in abalone like mollusks. Also, AbAIF-1 may involve in wound healing and shell repair after the tissue injury of abalone.

• 出版日期2010-8