Dual detection biosensor based on porous silicon substrate

作者：Simion Monica^*; Kusko Mihaela; Mihalache Iuliana; Bragaru Adina

来源：Materials Science and Engineering B-Solid State Materials for Advanced Technology, 2013, 178(19): 1268-1274.

DOI：10.1016/j.mseb.2013.03.003

• Nanoporous silicon
• Fluorescent spectroscopy
• Impedance spectroscopy

Due to the high surface-to-volume ratio (hundreds of m(2)/cm(3)) porous silicon became during the last years a good candidate material as substrate for biosensor application. Moreover, the versatility of surface chemistry allows different functionalization approaches and large number of molecules to be captured on well-defined areas. This paper reports a dual detection method for protein recognition processes developed on different nanostructured porous silicon (PS) substrates, based on using two complementary spectroscopic techniques: fluorescence and electrochemical impedance. The structures were tested for biomolecular recognition - biotin-strepavidin couples - in order to achieve an optimum surface for protein's immobilizations. Comparative analyses of the attachment degree and preservation of the biomolecules activity on the porous silicon surfaces and silicon slides are also described.

• 出版日期2013-11-20