DSMNC: a database of somatic mutations in normal cells

作者：Miao, Xuexia; Li, Xi; Wang, Lifei; Zheng, Caihong^*; Cai, Jun^*

来源：Nucleic Acids Research, 2019, 47(D1): D971-D975.

DOI：10.1093/nar/gky1045

• SINGLE-NUCLEOTIDE
• STEM-CELLS
• GENOME
• CANCER
• HETEROGENEITY
• AMPLIFICATION
• PLASMID

Numerous non-inherited somatic mutations, distinct from those of germ-line origin, occur in somatic cells during DNA replication per cell-division. The somatic mutations, recording the unique genetic cell-lineage history' of each proliferating normal cell, are important but remain to be investigated because of their ultra-low frequency hidden in the genetic background of heterogeneous cells. Luckily, the recent development of single-cell genomics biotechnologies enables the screening and collection of the somatic mutations, especial single nucleotide variations (SNVs), occurring in normal cells. Here, we established DSMNC: a database of somatic mutations in normal cells (http://dsmnc.big.ac.cn/), which provides most comprehensive catalogue of somatic SNVs in single cells from various normal tissues. In the current version, the database collected approximate to 0.8 million SNVs accumulated in approximate to 600 single normal cells (579 human cells and 39 mouse cells). The database interface supports the user-friendly capability of browsing and searching the SNVs and their annotation information. DSMNC, which serves as a timely and valuable collection of somatic mutations in individual normal cells, has made it possible to analyze the burdens and signatures of somatic mutations in various types of heterogeneous normal cells. Therefore, DSMNC will significantly improve our understanding of the characteristics of somatic mutations in normal cells.

• 出版日期2019-1-8
• 单位中国科学院大学; 中国科学院北京基因组研究所