Amphiphilic gamma-PGA nanoparticles administered on rat middle ear mucosa produce adjuvant-like immunostimulation in vivo

作者：Nilsson Johan S; Broos Sissela; Akagi Takami; Akashi Mitsuru; Hermansson Ann; Caye Thomasen Per; Lindstedt Malin; Greiff Lennart^*

来源：Acta Oto-Laryngologica, 2014, 134(10): 1034-1041.

DOI：10.3109/00016489.2014.918278

• Inflammation
• vaccination
• antigen delivery system

Conclusion: Amphiphilic biodegradable nanoparticles (NPs) composed of poly(g-glutamic acid) conjugated with L-phenylalanine ethylester (gamma-PGA-Phe NPs) applied on the rat middle ear mucosa produce an inflammatory type 1 response. The observation is of relevance for the use of gamma-PGA-Phe NPs as a concomitant antigen delivery system and adjuvant measure in the context of vaccinations. Objectives: To examine effects of topical mucosal administration of gamma-PGA-Phe NPs as a potentially combined antigen delivery system and adjuvant. Methods: gamma-PGA-Phe NPs were administered on rat middle ear mucosa in a sham-controlled design and the response was monitored, focusing on soluble markers in mucosal surface liquids and on overall histopathology. Results: gamma-PGA-Phe NPs produced a dose-and time-dependent inflammatory response characterized by generation of proinflammatory cytokines (IL-1 alpha, IL-1 beta, IL-6, MIP-1 alpha, and TNF-alpha) and associated histopathological changes.

• 出版日期2014-10