Black Tea Polyphenols Target Matrix Metalloproteinases, RECK, Proangiogenic Molecules and Histone Deacetylase in a Rat Hepatocarcinogenesis Model

作者：Murugan R Senthil; Vinothini G; Hara Y; Nagini S^*

来源：Anticancer Research, 2009, 29(6): 2301-2305.

• Angiogenesis
• black tea polyphenols
• chemoprevention
• hepatocellular carcinoma
• histone deacetylation
• invasion

Background: The aim of this study was to evaluate the chemopreventive effects of black tea polyphenols (Polyphenon-B) on markers of invasion and angiogenesis during dimethylaminoazobenzene (DAB)-induced hepatocarcinogenesis. Materials and Methods: Male Sprague-Dawley rats were divided into four groups. The rats in groups 1 and 2 were given 0.06% DAB in the diet for 3 months followed by the normal diet. The rats in group 2 received in addition 0.05% Polyphenon-B in the basal diet. The group 3 animals were given 0.05% Polyphenon-B alone in the basal diet. The group 4 animals served as the control. Results: The dietary administration of DAB induced well-differentiated hepatocellular carcinomas (HCC) that showed increased expression of the markers of invasion, angiogenesis and epigenetic histone deacetylation compared with the controls. The administration of Polyphenon-B significantly reduced the incidence of DAB-induced hepatomas as evidenced by modulation of the markers of invasion (matrix metalloproteinase, MMP-2, MMP-9, tissue inhibitor of matrix metalloproteinase, TIMP-2, and reversion-inducing cysteine rich protein with Kazal motifs RECK) and angiogenesis (hypoxia inducible factor 1 alpha, HIF1 alpha, vascular endothelial growth factor, VEGF, and VEGF receptor, VEGFR1) as well as the expression of histone deacetylase HDAC-1. Conclusion: The results of the present study provide evidence that Polyphenon-B has potential as a chemopreventive agent.

• 出版日期2009-6