Upregulation of miR-191 promotes cell growth and invasion via targeting TIMP3 in prostate cancer

作者：Wang, Xueling; Shi, Zhimin; Liu, Xiaoxia; Su, Ying; Li, Weixia; Dong, Haiping; Zhao, Liwei; Li, Manman; Wang, Yunxiao; Jin, Xiu; Huo, Zhongchao^*

来源：Journal of Buon, 2018, 23(2): 444-452.

• miR-191
• prostate cancer
• invasion
• TIMP3

Purpose: Prostate cancer (PCa) is the most frequently malignant neoplasm in men. MicroRNAs (miRs) have been identified to play important biological roles in a variety of tumors. Several studies showed that miR-191 was involved in the development of different cancers, but its role in prostate cancer remains unclear. @@@ Methods: Human PCa cell lines DU145, PC-3 and LN-CAP, and benign prostate hyperplasia (BPH) and human prostate epithelial cell line RWPE-1 were used. The expression level of miR-191 in 48 paired prostate tumor and adjacent normal tissues was assessed along with the clinical patient features. Synthetic miR-191 mimics and inhibitors were used to overexpress or inhibit the miR-191 level. CCK8 and colony formation assay were used to evaluate the cell growth. The ability of cell invasion was studied by transwell assay. Dual-luciferase experiment was used to identify the target gene and western blot was performed to evaluate the protein level. @@@ Results: miR-191 was overexpressed in PCa tissue samples compared to the normal group as well in PCa-derived cell lines. Upregulation miR-191 in PC-3 cells significantly promoted while downregulation miR-191 in DU145 cells retarded the cell proliferation and invasion. Furthermore, TIMP3 were proved to be a direct target gene of miR-191 and knockdown of TIMP3 reversed the function of miR-191 downregulation. @@@ Conclusion: This study demonstrated that miR-191 promoted the cell growth and invasion ability in prostate cancer through downregulating TIMP3 and might be a potential target for the biotherapy for PCa.

• 出版日期2018-3
• 单位河北工程大学