Dried blood spot sampling of nilotinib in patients with chronic myeloid leukaemia: a comparison with venous blood sampling

作者：Boons Christel C L M^*; Chahbouni Abdel; Schimmel Anneliene M; Wilhelm Abraham J; den Hartog Yvonne M; Janssen Jeroen J W M; Hendrikse N Harry; Hugtenburg Jacqueline G; Swart Eleonora L

来源：Journal of Pharmacy and Pharmacology, 2017, 69(10): 1265-1274.

DOI：10.1111/jphp.12757

• chronic myeloid leukaemia
• dried blood spot
• nilotinib
• therapeutic drug monitoring

ObjectivesTo compare nilotinib concentrations obtained by venous blood sampling and dried blood spot (DBS) in patients with chronic myeloid leukaemia (CML). It was investigated how to predict nilotinib plasma levels on the basis of DBS.
MethodsForty duplicate DBS and venous blood samples were collected from 20 patients. Capillary blood was obtained by finger prick and spotted on DMPK-C Whatman sampling paper, simultaneously with venous blood sampling. Plasma concentrations were predicted from DBS concentrations using three methods: (1) individual and (2) mean haematocrit correction and (3) the bias between plasma and DBS concentrations. Results were compared using Deming regression and Bland-Altman analysis.
Key findingsNilotinib plasma concentrations ranged from 376 to 2663g/l. DBS concentrations ranged from 144 to 1518g/l. The slope was 0.56 (95% CI, 0.51 to 0.61) with an intercept of -41.68g/l (95% CI, -93.78 to 10.42). Mean differences between calculated and measured plasma concentrations were -14.3% (method 1), -14.0% (method 2) and -0.6% (method 3); differences were within 20% of the mean in 73%, 85% and 80% of the samples, respectively. The slopes were respectively 0.96 (95% CI, 0.86 to 1.06), 0.95 (95% CI, 0.86 to 1.03) and 1.00 (95% CI, 0.91 to 1.09).
ConclusionsPlasma concentrations of nilotinib could be predicted on the basis of DBS. DBS sampling to assess nilotinib concentrations in CML patients seems a suitable alternative for venous sampling.

• 出版日期2017-10