Anticoagulant Activity of a Sulfated Polysaccharide Isolated from the Green Seaweed Caulerpa cupressoides

作者：Gurgel Rodrigues Jose Arievilo; Oliveira Vanderlei Edfranck de Sousa; Bessa Erika Facanha; Magalhaes Francisco de Araujo; Monteiro de Paula Regina Celia; Lima Vilma; Barros Benevides Norma Maria

来源：Brazilian Archives of Biology and Technology, 2011, 54(4): 691-700.

• marine algae
• sulfated macromolecules
• chemical analysis
• APTT test

The aim of this study was to evaluate certain molecular characteristics of a sulfated polysaccharide (SPs) with anticoagulant properties, isolated from Caulerpa cupressoides (Chlorophyta). Crude SPs were extracted by proteolytic digestion (papain), followed by ion-exchange chromatography on a DEAE-cellulose column. The fractions obtained were analyzed for molecular mass, 0.5% agarose gel electrophoresis and chemical composition. The activated partial thromboplastin time (APTT) test was applied using normal human plasma and standard heparin (HEP) (193 IU mg(-1)). The yield was similar to 3%, and the chromatography procedure separated the material into three different SP fractions (F I, F II and F III), eluted at the concentrations of 0.50, 0.75 and 1.00 M of NaCl, respectively. Only fraction F II was active (24.62 IU mg(-1)), with high sulfate content (23.79%) and number of molecular mass peaks. Therefore, the APTT of a fraction isolated from C. cupressoides was less potent than HEP.

• 出版日期2011-8