A novel method for sex determination of giant panda

作者：Xu Xiao; Wei Kun; Zhang Wen Ping; Lin Ling; Wang Xiao Fang; Shen Fu Jun; Zhang Liang; Zhang Zhi He; Yue Bi Song^*

来源：Annales Zoologici Fennici, 2007, 44(6): 401-404.

A fast, inexpensive, and reliable method for sex determination of giant panda was developed using amplification of giant panda zinc finger alleles (ZFX/ZFY). Two sets of primers targeted a conserved region in the exon of the ZFX/ZFY genes worked in a single PCR process, and provided positive results for both males and females. Sexes of individuals were revealed by sex-specific banding patterns (females: 447 bp, 146 bp; males: 447 bp, 350 bp, 146 bp). This method was successfully tested on blood sample from pandas of known sex. Non-invasive tests revealed that this method can also be applied to non-invasive samples such as hair or feces.

• 出版日期2007-12-19
• 单位四川大学