A RAPID BIOASSAY FOR RECOMBINANT INTERLEUKIN-22

作者：Xu Fei; Li Xiao Ying; Zhang Jun Yao; Xing Rui Qing; Li Jun Hong; Fu Qiang^*

来源：Journal of Immunoassay and Immunochemistry, 2010, 31(1): 71-78.

DOI：10.1080/15321810903405100

• bioassay
• HepG2
• interleukin-22 (IL-22)
• luciferase assay
• recombinant
• STAT3

Interleukin-22 is a novel cytokine produced mainly in activated T cells. The elaborate biological functions of IL-22 in vivo are still widely unknown. In this report, we describe a rapid, simple, and reproducible in vitro cell-based bioassay for measuring the bioactivity of recombinant interleukin-22 (IL-22) to study the primary function of IL-22 in vivo. Human hepatocyte cell line (HepG2) was transfected with pSTAT3-Luc, a plasmid carrying the luciferase gene under the control of STAT3. After screening and selection, one stable clone was established which generates a strong response to recombinant human IL-22 (rhIL-22) stimulation in a dose-dependent manner. The cell showed ED50 of 17.0 +/- 1.4 ng/mL (n = 15) to recombinant human IL-22. Pre-incubation of anti-IL-22 mAb with IL-22 recombinant proteins completely blocked the bioactivities. The assay can be completed within one day. The current assay provides a rapid analytical method to measure the biological activity of IL-22 in vitro.

• 出版日期2010
• 单位四川大学