New molecular features of cowpea bean (Vigna unguiculata, l. Walp) beta-vignin

作者：Ferreira Ederlan de Souza; Capraro Jessica^*; Sessa Fabio; Magni Chiara; Demonte Aureluce; Consonni Alessandro; Neves Valdir Augusto; Cilli Eduardo Maffud; Duranti Marcello; Scarafoni Alessio

来源：Bioscience Biotechnology and Biochemistry, 2018, 82(2): 285-291.

DOI：10.1080/09168451.2017.1419855

• Vigna ssp
• vicilin proteins
• beta-vignin
• glycosylation
• metal ion binding

Cowpea seed -vignin, a vicilin-like globulin, proved to exert various health favourable effects, including blood cholesterol reduction in animal models. The need of a simple scalable enrichment procedure for further studies for tailored applications of this seed protein is crucial. A chromatography-independent fractionation method allowing to obtain a protein preparation with a high degree of homogeneity was used. Further purification was pursued to deep the molecular characterisation of -vignin. The results showed: (i) differing glycosylation patterns of the two constituent polypeptides, in agreement with amino acid sequence features; (ii) the seed accumulation of a gene product never identified before; (iii) metal binding capacity of native protein, a property observed only in few other legume seed vicilins.

• 出版日期2018