Soyasaponins Aa and Ab Exert an Anti-Obesity Effect in 3T3-L1 Adipocytes Through Downregulation of PPAR gamma

作者：Yang Seung Hwan; Ahn Eun Kyung; Lee Jung A; Shin Tai Sun; Tsukamoto Chigen; Suh Joo won^*; Mei Itabashi; Chung Gyuhwa

来源：Phytotherapy Research, 2015, 29(2): 281-287.

DOI：10.1002/ptr.5252

• soyasaponins
• adipogenesis
• peroxisome proliferator-activated receptor gamma (PPAR)
• adipogenic marker genes
• 3T3-L1 cells

Saponins are a diverse group of biologically functional products in plants. Soyasaponins are usually glycosylated, which give rise to a wide diversity of structures and functions. In this study, we investigated the effects and molecular mechanism of soyasaponins Aa and Ab in regulating adipocyte differentiation and expression of adipogenic marker genes in 3T3-L1 adipocytes. Soyasaponins Aa and Ab dose-dependently inhibited the accumulation of lipids and the expression of adiponectin, adipocyte determination and differentiation factor 1/sterol regulatory element binding protein 1c, adipocyte fatty acid-binding protein 2, fatty acid synthase, and resistin in 3T3-L1 adipocytes. In addition, soyasaponins Aa and Ab suppressed the transcriptional activity of peroxisome proliferator-activated receptor (PPAR) in HEK 293T cells. Furthermore, we confirmed that the expression of PPAR and of CCAAT-enhancer-binding protein (C/EBP) was suppressed at both the mRNA and protein levels in 3T3-L1 adipocytes by treatment with soyasaponins Aa and Ab. Taken together, these findings indicate that soyasaponin Aa and Ab markedly inhibit adipocyte differentiation and expression of various adipogenic marker genes through the downregulation of the adipogenesis-related transcription factors PPAR and C/EBP in 3T3-L1 adipocytes.

• 出版日期2015-2