A novel antimicrobial peptide from skin secretions of the earthworm, Pheretima guillelmi (Michaelsen)

作者：Li, Wenliang; Li, Sisi; Zhong, Jian; Zhu, Zhu; Liu, Jingze^*; Wang, Wenhong

来源：Peptides, 2011, 32(6): 1146-1150.

DOI：10.1016/j.peptides.2011.04.015

• Earthworm
• Antimicrobial peptide
• Skin secretion
• Lumbricin
• Pheretima guillelmi

A novel lumbricin-like antimicrobial peptide named lumbricin-PG was isolated from skin secretions of the earthworm, Pheretima guillelmi (Michaelsen), using a procedure of one step Sephadex G-50 gel filtration and one step C(8) reverse-phase high-performance liquid chromatography (RP-HPLC). Its amino acid sequence was determined as FSRYARMRDSRPWSDRKNNYSGPQFTYPPEKAPPEKLIKWNN EGSPIFEMPAEGGHIEP by Edman degradation combined with cDNA cloning and mass spectrometry analysis. The cDNA encoding lumbricin-PG was cloned by cDNA library screening. The predicted protein from the cDNA sequence was composed of 73 amino acid residues including a mature lumbricin-PG and predicted signal peptide. It showed similarity with lumbricin antimicrobial peptide from the earthworm, Lumbricus rubellus by BLAST search. Purified lumbricin-PG exerted potential antimicrobial activities against bacteria and fungi; it showed weak hemolysis activity against human and rabbit red cells.

• 出版日期2011-6
• 单位河北师范大学; 昆明医科大学; 东南大学