Development of a multiplex PCR assay to detect Edwardsiella tarda, Streptococcus parauberis, and Streptococcus iniae in olive flounder (Paralichthys olivaceus)

作者：Park Seong Bin; Kwon Kyoung; Cha In Seok; Jang Ho Bin; Nho Seong Won; Fagutao Fernand F; Kim Young Kyu; Yu Jong Earn; Jung Tae Sung^*

来源：Journal of Veterinary Science, 2014, 15(1): 163-166.

DOI：10.4142/jvs.2014.15.1.163

• Edwardsiella tarda
• multiplex PCR
• olive flounder
• Streptococcus iniae
• Streptococcus parauberis

A multiplex PCR protocol was established to simultaneously detect major bacterial pathogens in olive flounder (Paralichthys olivaceus) including Edwardsiella (E.) tarda,;Streptococcus (S.) parauberis, and S. iniae. The PCR assay was able to detect 0.01 ng of E. tarda, 0.1 ng of S. parauberis, and 1 ng of S. iniae genomic DNA. Furthermore, this technique was found to have high specificity when tested with related bacterial species. This method represents a cheaper, faster, and reliable alternative for identifying major bacterial pathogens in olive flounder, the most important farmed fish in Korea.

• 出版日期2014-3