Associations between long-term exposure to PM2.5 component species and blood DNA methylation age in the elderly: The VA normative aging study

作者：Nwanaji Enwerem Jamaji C^*; Dai Lingzhen; Colicino Elena; Oulhote Youssef; Di Qian; Kloog Itai; Just Allan C; Hou Lifang; Vokonas Pantel; Baccarelli Andrea A; Weisskopf Marc G; Schwartz Joel D

来源：Environment International, 2017, 102: 57-65.

DOI：10.1016/j.envint.2016.12.024

• DNA methylation age
• Particulate matter 2.5
• Long-term exposure
• Epigenetics

Background: Long-term PM2.5 exposure and aging have been implicated in multiple shared diseases; studying their relationship is a promising strategy to further understand the adverse impact of PM2.5 on human health.
Objective: We assessed the relationship of major PM2.5 component species (ammonium, elemental carbon, organic carbon, nitrate, and sulfate) with Horvath and Hannum DNA methylation (DNAm) age, two DNA methylation-based predictors of chronological age.
Methods: This analysis included 552 participants from the Normative Aging Study with multiple visits between 2000 and 2011 (n = 940 visits). We estimated 1-year PM2.5 species levels at participants' addresses using the GEOS-chem transport model. Blood DNAm-age was calculated using CpG sites on the Illumina HumanMethylation450 BeadChip. We fit linear mixed-effects models, controlling for PM2.5 mass and lifestyle/environmental factors as fixed effects, with the adaptive LASSO penalty to identify PM2.5 species associated with DNAm-age.
Results: Sulfate and ammonium were selected by the LASSO in the Horvath DNAm-age models. In a fully-adjusted multiple-species model, interquartile range increases in both 1-year sulfate (95%CI: 0.28, 0.74, P < 0.0001) and ammonium (95%CI: 0.02, 0.70, P = 0.04) levels were associated with at least a 0.36-year increase in Horvath DNAm-age. No PM2.5 species were selected by the LASSO in the Hannum DNAm-age models. Our findings persisted in sensitivity analyses including only visits with 1-year PM2.5 levels within US EPA national quality standards.
Conclusion: Our results demonstrate that sulfate and ammonium were most associated with Horvath DNAm-age and suggest that DNAm-age measures differ in their sensitivity to ambient particle exposures and potentially disease. Published by Elsevier Ltd.

• 出版日期2017-5
• 单位西北大学