Association between STAT1 activity and BRAF mutations in papillary thyroid carcinomas

作者：Yim Ji Hye; Kim Eun Sook^*; Choi Hyun Jung; Jeon Min Ji; Han Ji Min; Kim Won Gu; Kim Tae Yong; Gong Gyungub; Kim Sang Yoon; Kim Won Bae; Shong Young Kee

来源：Journal of Surgical Oncology, 2012, 106(6): 719-723.

DOI：10.1002/jso.23125

• papillary thyroid carcinoma
• STAT1 transcription factor
• BRAF mutation
• thyroid neoplasm

Background Signal transducer and activator of transcription-1 (STAT1) plays a critical role in tumorigenesis by controlling several functions in both tumor cells and the immune system, and is considered to be a tumor suppressor. The present study evaluated the activity of STAT1 in human papillary thyroid carcinomas (PTC). Methods STAT1 activity was measured in nuclear extracts of tumor tissues from 35 PTC patients using an ELISA-based kit. Results STAT1 activity was significantly lower in tumors than in the surrounding normal thyroid tissues (P?%26lt;?0.01). The association between STAT1 activity and clinicopathologic factors was analyzed in PTC tissues. STAT1 activity was significantly lower in tumors that measured 2?cm or more than in tumors that measured 2?cm or less (P?=?0.044). Moreover, tumor size was inversely correlated with STAT1 activity (Spearman%26apos;s rank correlation coefficient (rho)?=?-0.34, P?=?0.048). In addition, tumors with BRAF mutations showed lower STAT1 activity than wild-type BRAF tumors [0.064 (0.0560.124) vs. 0.108 (0.0730.153) arbitrary units, P?=?0.048]. Conclusion STAT1 activity is suppressed in PTCs (as measured by DNA-binding activities). The tumor with T1799A BRAF mutation and tumor sizes of 2?cm or more were clinicopathologic parameters associated with lower STAT1 activity. STAT1 activity of tumor might be one of potential biomarkers for PTC%26apos;s. J. Surg. Oncol. 2012; 106:719723.

• 出版日期2012-11