Anti-angiogenic Function of a Korean Ginseng and Toad Venom Complex, Doksamsumsu-dan (DSSSD) Analyzed by a Forwarded Phase Antibody Microarray

作者：Kang Hwajung; Park Byung Rok; Yoo Hwa Seung; Kwon Ki Rok; Kang In Cheol^*

来源：BioChip Journal, 2015, 9(3): 222-231.

DOI：10.1007/s13206-015-9308-6

• Angiogenesis
• DSSSD
• Antibody microarray
• HUVEC
• CAM

We investigated anti-angiogenic effects of Korean Ginseng and Toad venom complex, Doksamsumsu-dan (DSSSD) using a Forwarded Phase Protein Array (FPPA) technology. DSSSD significantly inhibited bFGF-induced the proliferation and migration of human umbilical vein endothelial cell (HUVEC)s in a dose-dependent manner FACS analysis was carried out to confirm this inhibition by demonstrating increase of apoptosis in HUVECs treated with DSSSD. The cell apoptosis was due to stimulating Bid expression by western blotting analysis. Blood vessel formation in a chick chorioallantoic membrane (CAM) treated with DSSSD was markedly suppressed compared with a PBS-treated control group. To investigate more detail the mode-of-action mechanism of DSSSD, we used a FPPA-based antibody microarray to analyze differential expression profiling of cellular signaling proteins. We found up-regulated expression of SAM 68, a RNA-binding protein known to be involved in cell proliferation, migration, and apoptosis. These results suggest that inhibition of angiogenesis by DSSSD may be one of anti-cancer effects of DSSSD.

• 出版日期2015-9-20