Protective Effects of Hypothalamic Proline-Rich Peptide and Cobra Venom Naja Naja Oxiana on Dynamics of Vestibular Compensation Following Unilateral Labyrinthectomy

作者：Galoyan Armen A; Khalaji Naser; Hambardzumyan Lilja E; Manukyan Larisa P; Meliksetyan Irina B; Chavushyan Vergine A; Sarkisian Vaghinak H^*; Sarkissian John S

来源：Neurochemical Research, 2010, 35(11): 1747-1760.

DOI：10.1007/s11064-010-0239-7

• Hypothalamic proline-rich peptide (PRP-1)
• Cobra venom (NOX)
• Deiters' nucleus
• Hypothalamic paraventricular and supraoptic nuclei
• Tetanic-posttetanic potentiation and depression
• Acidic phosphatase activity

We tested the action of proline-rich peptide (PRP-1) and cobra venom Naja Naja Oxiana (NOX) on Deiters' nucleus neurons at 3rd, 15th and 35th days after unilateral labyrinthectomy (UL). Early and late tetanic, post-tetanic potentiation and depression of Deiters'neurons to bilateral high frequency stimulation of hypothalamic supraoptic and paraventricualar nuclei was studied. The analysis of spike activity was carried out by mean of on-line selection and special program. The complex averaged peri-event time and frequency histograms shows the increase of inhibitory and excitatory reactions of Deiters' neurons at early stage of vestibular compensation following PRP-1 and NOX injection, reaching the norm at the end of tests. In histochemical study the changes in Ca(2+)-dependent acidic phosphatase (AP) activity in neurons was discovered. It was shown that in UL animals the total disappearance or delay of decolorizing of Deiters' neurons lead to neurodegenerative pattern as cellular "shade". AP activity after UL and PRP-1 injection exerts more effective recovery of neurons in comparison with events, observed after the administration of NOX. The data of this study indicate that PRP-1 and NOX are protectors, which may successfully recover the disturbed vestibular functions.

• 出版日期2010-11