A 25 bp-long insertional mutation in the BmVarp gene causes the waxy translucent skin of the silkworm, Bombyx mori

作者：Ito Katsuhiko; Katsuma Susumu; Yamamoto Kimiko; Kadono Okuda Keiko; Mita Kazuei; Shimada Toru^*

来源：Insect Biochemistry and Molecular Biology, 2009, 39(4): 287-293.

DOI：10.1016/j.ibmb.2009.01.005

• Positional cloning
• ow
• Vacuolar sorting protein
• Ankyrin repeat
• Varp

In Bombyx mori, there are more than 35 mutant strains whose larval skin color is transparent. The waxy translucent strain ow is one of the oily mutants which lack accumulation of uric acid in the epidermis. Here we performed positional cloning of the ow gene using the Bombyx draft genome sequence. For fine structure mapping, we succeeded to narrow the ow linked region to approximately 150 kb. and identified the ow candidate gene by annotation analysis and DNA sequencing. The complete cDNA sequences of the ow gene from wild-type strains were 3501 bp-long and potentially encoded a protein of 920 amino acids. We found a 25 bp-long insertion in this gene in the ow mutant strain, resulting in a frame-shift mutation and generation of a premature stop codon. A BLAST search revealed that this protein had high homology to Varp, a recently identified protein containing a vacuolar sorting protein 9 domain and ankyrin repeats, and we termed the silkworm protein BmVarp. Varp has been shown to regulate endosome dynamics, suggesting that BmVarp may play an important role in the incorporation and/or accumulation of uric acid in the epidermis.

• 出版日期2009-4