Acceleration of protein decomposition by photocatalytic Ti(IV)-doped calcium hydroxyapatite particles and its application for reduction of pathogenic proteins

作者：Kandori Kazuhiko^*; Wada Hiroyuki; Wakamura Masato

来源：Colloid and Polymer Science, 2014, 292(9): 2261-2268.

DOI：10.1007/s00396-014-3256-2

• Ti(IV)-doped calcium hydroxyapatite
• Photocatalytic activity
• Decomposition of protein
• Bovine serum albumin
• Lysozyme
• Reduction of pathogenic protein

Acceleration of protein decomposition from single- and mixed-protein solutions on the surface of Ti(IV)-doped calcium hydroxyapatite (TiHap) particles with a Ti/(Ca+Ti) atomic ratio (X (Ti)) of 0.10 and 0.20 under ultraviolet (UV) irradiation was investigated. The UV irradiation started immediately after dispersing the TiHap particles in protein solution in a quartz tube (0 h UV method). Lysozyme (LSZ) was steeply decomposed in a LSZ single system by the 0 h UV method. Furthermore, a selective photocatalytic decomposition of LSZ was observed on the mixed-protein system; i.e., only LSZ molecules were decomposed completely from the bovine serum albumin (BSA; 2.5 mg/cm(3)) -aEuro parts per thousand LSZ (1.0 mg/cm(3)) mixture using TiHap particles. The selective decomposition of pathogenic protein beta(2)-microglobulin (beta(2)-MG) from 20 mu g/cm(3) beta(2)-MG -aEuro parts per thousand 10 mg/cm(3) BSA mixture in 1.5 mL x 10(-4) M KCl solution was also examined. The UV irradiation started at 24 h after dispersing TiHap particles in BSA-beta(2)-MG-mixed solution for attaining the protein adsorption equilibrium (24 h UV method). It was revealed that beta(2)-MG molecules were entirely destructed by the 24 h UV method by only irradiating UV light to the dispersions for 24 h. The obtained selective decomposition of beta(2)-MG strongly suggested that TiHap particles can be applied for a blood purification therapy using UV irradiation.

• 出版日期2014-9