Microsporidial infections due to Encephalitozoon intestinalis in non-HIV-infected patients with chronic diarrhoea

作者：Yakoob J^*; Abbas Z; Beg M Asm; Jafri W; Naz S; Khalid A; Khan R

来源：Epidemiology and Infection, 2012, 140(10): 1773-1779.

DOI：10.1017/S0950268811002639

• Chronic diarrhoea
• Encephalitozoon intestinalis
• modified trichrome staining
• PCR

We determined the prevalence of microsporidia Enterocytozoon (Ent.) bieneusi and Encephalitozoon (E.) intestinalis infection in patients with chronic diarrhoea and hepatocellular carcinoma (HCC). A total of 330 stool samples were examined from 171 (52%) patients with chronic diarrhoea, 18(5%) with HCC while 141 (43%) were controls. Stool microscopy, polymerase chain reaction (PCR) with species-specific primers for Ent. bieneusi and E. intestinalis and sequencing were carried out. Microsporidia were found by trichrome staining in 11/330 (3 %) and E. intestinalis by PCR in 13/330 (4%) while Ent. bieneusi was not detected. PCR for E. intestinalis was positive in 8/171 (5%) stool samples from patients with chronic diarrhoea, 2/141 (1.4%) samples from healthy controls and in 3/18(17%) samples from patients with HCC. In the chronic diarrhoea group, E. intestinalis was positive in 4/171 (2.3%) (P = 069) stool samples compared to 2/18 (11%) (P = 006) in the HCC group and 2/141 (1.4%) from healthy controls. E. intestinalis infection was significantly associated with chronic diarrhoea and HCC in these patients who were negative for HIV. Stool examination with trichrome or species-specific PCR for microsporidia may help establish the cause of chronic diarrhoea.

• 出版日期2012-10