Agrobacterium tumefaciens-Mediated Transformation of the Vegetative Dikaryotic Mycelium of the Cultivated Mushroom Flammulina velutipes

作者:Okamoto Tomomitsu; Yamada Masato; Sekiya Shuichi; Okuhara Toru; Taguchi Goro; Inatomi Satoshi; Shimosaka Makoto*
来源:Bioscience Biotechnology and Biochemistry, 2010, 74(11): 2327-2329.
DOI:10.1271/bbb.100398

摘要

Agrobacterium tumefaciens was used to transform the vegetative dikaryotic mycelium of Flammulina velutipes using a hygromycin B resistance gene as selectable marker. The gene coding for urogen III methyltransferase (cob) was introduced into F. velutipes dikaryotic cells. The resulting transformant cells generated a bright red fluorescence, indicating that cob is promising as a reporter gene in F. velutipes.

  • 出版日期2010-11