Agaricus blazei extract attenuates rotenone-induced apoptosis through its mitochondrial protective and antioxidant properties in SH-SY5Y neuroblastoma cells

作者：Gobi Veerappan Venkatesh; Rajasankar Srinivasagam; Ramkumar Muthu; Dh****akshmi Chinnasamy; Manivasagam Thamilarasan; Thenmozhi Arokiasamy Justin; Essa Musthafa Mohamed; Chidambaram Ranganathan

来源：Nutritional Neuroscience, 2018, 21(2): 97-107.

DOI：10.1080/1028415X.2016.1222332

• A. blazei
• Rotenone
• Oxidative stress
• Mitochondria membrane potential
• Apoptosis

The present study was aimed to find out the effect of Agaricus blazei mushroom extract against rotenone-induced cellular model. SH-SY5Y neuroblastoma cells are divided into four experimental groups (control, rotenone (100 nM), A. blazei (5 mu g/ml) + rotenone (100 nM), and A. blazei alone treated) based on MTT assay, cells were allowed to measure the ROS, TBARS levels, and antioxidants activities. Finally, mitochondrial transmembrane potential (MMP) and expressions of apoptotic proteins were also analyzed. Pre-treatment with A. blazei significantly enhanced cell viability, attenuated rotenone-induced ROS, MMP, and apoptosis. Our results indicated that anti-apoptotic properties of this natural compound due to its antioxidant and mitochondrial protective function protect rotenone-induced cytotoxicity. Therefore, it may be concluded that A. blazei can be further developed as a promising drug for the treatment of Parkinson's disease (PD).

• 出版日期2018
• 单位中国医学科学院北京协和医院