Preparation of C-13-labelled cis-zearalenone and its application as internal standard in stable isotope dilution analysis

作者：Drzymala S^*; Riedel J; Koeppen R; Garbe L A; Koch M

来源：World Mycotoxin Journal, 2014, 7(1): 45-52.

DOI：10.3920/WMJ2013.1610

• zearalenone
• isomerisation
• occurrence
• edible oil
• SIDA
• HPLC-MS/MS
• quantification

Pure U-[C-13(18)]-labelled cis-zearalenone (cis-ZEA) has been prepared and characterised as internal standard (ISTD) for a reliable quantification of cis-ZEA in contaminated food and feed products. The cis-isomer of the naturally trans-configurated Fusarium mycotoxin zearalenone is often neglected. However, isomerisation easily occurs by exposure of ZEA to (UV-) light. Thus, the applicability of the new cis-ZEA ISTD was demonstrated in a long-term isomerisation study comparing naturally trans-ZEA-contaminated edible oil with spiked edible oil. To estimate the benefits of the newly prepared cis-ZEA ISTD, various approaches to quantify cis-ZEA by high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) were compared. As a result, a significant bias was revealed if no appropriate cis-ZEA standards are used. Furthermore, the new ISTD was applied to the analysis of 15 edible oils by stable isotope dilution analysis in combination with HPLC-electrospray ionisation-MS/MS. One of the maize germ oils showed the presence of cis-ZEA above LOD (> 0.3 mu g/kg), whereas two out of 15 maize germ oils were found to be contaminated with trans-ZEA (range 17.0-31.0 mu g/kg).

• 出版日期2014-2