Application and Equivalence Assessment for Determining Ethamsylate by Using Potassium Ferricyanide as Spectroscopic Probe Reagent

作者：Liu, Litao; Li, Jing; Li, Quanmin^*

来源：Analytical Sciences, 2010, 26(11): 1163-1167.

DOI：10.2116/analsci.26.1163

• RANDOMIZED CONTROLLED-TRIAL
• PHARMACEUTICAL-PREPARATIONS

In this paper, a novel method has been established to determine ethamsylate using potassium ferricyanide as a spectroscopic probe reagent. It has been demonstrated that Fe(III) is reduced to Fe(II) by ethamsylate, and that the formed Fe(II) reacts with potassium ferricyanide to form soluble prussian blue (KFeIII[Fe-II(CN)(6)]). Beer's law is obeyed in the range of 0.16 - 24.00 mu g mL(-1) with the molar absorption coefficient of 2.1 x 10(4) L mol(-1) cm(-1). The detection limit (3 sigma/k) is 0.11 mu g mL(-1). This method has been successfully applied to determine ethamsylate in pharmaceutical and serum samples with satisfactory results, and presented quite satisfactory credibility during method equivalence assessment.

• 出版日期2010-11
• 单位河南师范大学