Affinity Isolation of Desired Restriction Fragment from Human Genome Using Double-duplex Invasion of Biotin-bound Pseudo-complementary PNA

作者：Shigi Narumi^*; Rajendran Arivazhagan; Wang Xiaohui; Kunifuda Hiroko; Sumaoka Jun; Komiyama Makoto

来源：Chemistry Letters, 2015, 44(11): 1569-1571.

DOI：10.1246/cl.150682

A predetermined fragment in the human genome was successfully isolated by affinity separation using biotin-streptavidin interaction. The whole genome from human cells was first digested by restriction enzymes and then treated with a pair of pseudo-complementary peptide nucleic acids (pcPNAs). A biotin was covalently bound to either of the pcPNAs. With the use of streptavidin-coupled resin, the targeted fragment involving the invasion site of the pcPNAs was selectively picked up from the genomic digests in high yields.

• 出版日期2015-11-5