Azobenzene-modified antisense oligonucleotides for site-specific cleavage of RNA with photocontrollable property

作者：Wang, Xingyu; Liang, Xingguo^*

来源：RSC Advances, 2016, 6(96): 93398-93402.

DOI：10.1039/c6ra20954h

• ESCHERICHIA-COLI
• GENE-EXPRESSION
• RNA/DNA HYBRID
• TETHERED DNA
• SEQUENCE
• H1
• PHOTOREGULATION
• TRANSCRIPTION
• BEARING
• DUPLEX

We presented a RNase H-based strategy for site-specific RNA cleavage using azobenzene-modified antisense oligonucleotides (ASO). The results showed that RNase H can bind to the ASO-RNA heteroduplex, and cut the RNA strand at predetermined sites. Moreover, cleavage rate can be regulated by photoirradiation, with a 5-fold photomodulation efficiency at most.

• 出版日期2016
• 单位陕西师范大学; 中国海洋大学