Dispersive liquid-liquid microextraction combined with ultra-high performance liquid chromatography for the simultaneous determination of 25 sulfonamide and quinolone antibiotics in water samples

作者：Herrera Herrera Antonio V; Hernandez Borges Javier^*; Borges Miquel Teresa M; Angel Rodriguez Delgado Miguel

来源：Journal of Pharmaceutical and Biomedical Analysis, 2013, 75: 130-137.

DOI：10.1016/j.jpba.2012.11.026

• Antibiotics
• Dispersive liquid-liquid microextraction
• Quinolones
• Sulfonamides
• Ultra-high performance liquid chromatography
• Water

In this work, a dispersive liquid-liquid microextraction (DLLME) procedure combined with ultra-high performance liquid chromatography with diode-array detection was developed to determine 25 antibiotics in mineral and run-off waters. Optimum DLLME conditions (5 mL of water at pH = 7.6, 20% (w/v) NaCl, 685 mu L of CHCl3 as extractant solvent, and 1250 mu L of ACN as disperser solvent) allowed the repeatable, accurate and selective determination of 11 sulfonamides (sulfanilamide, sulfacetamide, sulfadiazine, sulfathiazole, sulfadimidin, sulfamethoxypyridazine, sulfadoxine, sulfamethoxazole, sulfisoxazole, sulfadimethoxine and sulfaquinoxaline) and 14 quinolones (pipemidic acid, marbofloxacin, fleroxacin, levofloxacin, pefloxacin, ciprofloxacin, lomefloxacin, danofloxacin, enrofloxacin, sarafloxacin, difloxacin, moxifloxacin, oxolinic acid and flumequine). The method was validated by means of the obtention of calibration curves of the whole method as well as a recovery study at two levels of concentration. The LODs of the method were in the range 0.35-10.5 mu g/L with recoveries between 78% and 117%.

• 出版日期2013-3-5