First phylogenetic analysis of Avipoxvirus (APV) in Brazil

作者：Kunert Filho Hiran C; Cibulski Samuel P; Finkler Fabrine; Grassotti Tiela T; Jaenisch Fatima R F; de Brito Kelly C T; Carvalho Daiane; Lovato Maristela; de Brito Benito G

来源：Pesquisa Veterinaria Brasileira, 2016, 36(5): 357-362.

DOI：10.1590/S0100-736X2016000500001

• Turkey
• Poxviridae
• Avipoxvirus
• APV
• PCR
• phylogenetic analysis

This study represents the first phylogenetic analysis of avian poxvirus recovered from turkeys in Brazil. The clinical disorders related to fowlpox herein described occurred in a turkey housing system. The birds displaying characteristic pox lesions which were observed on the neck, eyelids and beak of the turkeys. Four affected turkeys were randomly chosen, euthanized and necropsied. Tissues samples were submitted for histopathological analysis and total DNA was further extracted, amplified by conventional PCR, sequenced and phylogenetically analyzed. Avian poxviruses specific PCR was performed based on P4b core protein gene sequence. The histological analysis revealed dermal inflammatory process, granulation tissue, hyperplasia of epithelial cells and inclusion bodies. The P4b gene was detected in all samples. Sequencing revealed a 100% nucleotide and amino acid sequence identity among the samples, and the sequences were deposited in GenBank (R). The four Avian poxviruses fragments sequenced in this study clustered along the A1 clade of avipoxviruses, and were classified as Avipoxvirus (APV). Additional studies, such as virus isolation, PCR and sequencing includinga large number of specimens from the Brazilian turkey production must be conducted due to the hazardous risk that poxvirus infections may cause to the Brazilian poultry production scenario, given that Brazil's turkey production attracts attention due to its economic importance worldwide. Our findings point to the need to identify the prevalence of APV in Brazilian turkey production, to perform risk assessment studies and continued surveillance of APV infections in both wild and commercial avian species.

• 出版日期2016-5