Azacitidine differentially affects CD4(pos) T-cell polarization in vitro and in vivo in high risk myelodysplastic syndromes

作者：Bontkes Hetty J^*; Ruben Jurjen M; Alhan Canan; Westers Theresia M; Ossenkoppele Gert J; van de Loosdrecht Arjan A

来源：Leukemia Research, 2012, 36(7): 921-930.

DOI：10.1016/j.leukres.2012.03.026

• Azacitidine
• Cytokines
• Regulatory T cells
• IL-17
• CD4 T-helper cells
• MDS

CD4(pos) T-cell subsets play a role in myelodysplastic syndromes (MDS) pathogenesis and may be affected upon 5-azacitidine (Aza) treatment. Aza enhanced human T(H)1 frequencies in vitro but not in vivo. The proportion of functional FoxP3(pos) regulatory T cells (Treg) was enhanced by Aza in vitro (p%26lt;0.0002), and a modest, temporary increase was observed in vivo (p=0.08). The overall number of T(H)17 was reduced both in vitro (p%26lt;0.03) and in vivo (p%26lt;0.006), indicating that Aza directly affects CD4(pos) polarization during activation in vitro. Upon in vivo treatment in high risk MDS patients, particularly the T(H)17-Treg axis is affected.

• 出版日期2012-7