A comprehensive evaluation of mixed mode interactions of HEA and PPA HyperCel (TM) chromatographic media

作者：Pezzini J; Cabanne C; Gantier R; Janakiraman V N; Santarelli X^*

来源：Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2015, 976: 68-77.

DOI：10.1016/j.jchromb.2014.11.020

• Mixed mode chromatography
• HEA HyperCel (TM)
• PPA HyperCel (TM)
• Purification
• Proteins

Mixed mode or multimodal chromatography has been developed for rational use of multiple interactions in a controlled manner, in contrast to non-specific interactions. Indeed, as the term "mixed mode" suggests, these resins allow different types of interactions within a single chromatographic medium. In this paper, HEA HyperCel (TM), PPA HyperCel (TM) mixed-mode chromatographic media have been studied. These mixed-mode sorbents typically involve hydrophobic pseudo-affinity interactions for binding and essentially ionic interactions (charge repulsion) for elution. We identified and characterized these different interactions in chromatographic experiments by exploiting specific properties of proteins using protein standards and complex mixtures. We highlighted the major intervention of at least two types of interactions in these media: hydrophobic and electrostatic interactions. We observed the behaviour of these resins at different pH, ionic strength, with different salts and buffers types and in the presence of different organic compounds.

• 出版日期2015-1-22