Limited Immunogenicity of HIV CD8(+) T-Cell Epitopes in Acute Clade C Virus Infection

作者：Radebe Mopo; Nair Kriebashnie; Chonco Fundisiwe; Bishop Karen; Wright Jaclyn K; van der Stok Mary; Bassett Ingrid V; Mncube Zenele; Altfeld Marcus; Walker Bruce D; Ndung'u Thumbi^*

来源：Journal of Infectious Diseases, 2011, 204(5): 768-776.

DOI：10.1093/infdis/jir394

Background. Human immunodeficiency virus type 1 (HIV-1)-specific CD8(+) responses contribute to the decline in acute peak viremia following infection. However, data on the relative immunogenicity of CD8(+) T-cell epitopes during and after acute viremia are lacking.
Methods. We characterized CD8(+) T-cell responses in 20 acutely infected, antiretroviral-naive individuals with HIV-1 subtype C infection using the interferon-gamma enzyme-linked immunosorbent spot assay. Eleven of these had not fully seroconverted at the time of analysis. Viruses from plasma were sequenced within defined cytotoxic T-lymphocyte (CTL) cell epitopes for selected subjects.
Results. At approximately 28 days after estimated initial infection, CD8(+) T-cell responses were directed against an average of 3 of the 410 peptides tested (range, 0-6); 2 individuals had no detectable responses at this time. At 18 weeks, the average number of peptides targeted had increased to 5 (range 0-11). Of the 56 optimal Gag CTL epitopes sequenced, 31 were wild-type in the infecting viruses, but only 11 of 31 elicited measurable CD8(+) T-cell responses.
Conclusions. These data demonstrate that the majority of CD8(+) responses are not elicited during acute HIV infection despite the presence of the cognate epitope in the infecting strain. There is a need to define factors that influence lack of induction of effective immune responses and the parameters that dictate immunodominance in acute infection.

• 出版日期2011-9-1