Application of water-soluble nanofilters for collection of airborne Mycobacterium tuberculosis DNA in hospital wards

作者：Vladimirsky M A; Shipina L K; Makeeva E S; Alyapkina Y S; Mikheev A Y; Morozov V N^*

来源：Journal of Hospital Infection, 2016, 93(1): 100-104.

DOI：10.1016/j.jhin.2016.02.009

• Aerosol
• Mycobacteria detection
• Polymerase chain reaction
• Water-soluble filters

A simple inexpensive technique for collection of airborne biomarkers of nosocomial infections is described. Biomarkers were collected on water-soluble electrospun nanofilters attached to a household vacuum cleaner from 6-10m(3) of air in 10-15 min within several wards of a tuberculosis clinic. Filters were then dissolved in water and tested for the presence of the IS6110 and regX3 genes of Mycobacterium tuberculosis (MTB) using real-time polymerase chain reaction. It was demonstrated that trace amounts of airborne MTB DNA (> 3 gene copies/m(3)) were always present in the air and on the surfaces in the wards occupied with tuberculosis patients having positive smear tests.

• 出版日期2016-5