An improved synthesis of disulfides linked beta-cyclodextrin dimer and its analytical application for dequalinium chloride determination by spectrofluorimetry

作者：Tang B^*; Liang HL; Xu KH; Mao Z; Shi XF; Chen ZZ

来源：Analytica Chimica Acta, 2005, 554(1-2): 31-36.

DOI：10.1016/j.aca.2005.08.048

• synthesis
• beta-cyclodextrin dimer
• disulfides
• dequalinium chloride (DQC)
• spectrofluorimetry

An improved synthesis of beta-cyclodextrin (beta-CD) dimer, containing two P-CD moieties that are linked through their sides by disulfides was presented. The dimer was characterized by means of IR,H-1 NMR and elemental analysis. The inclusion complexation behavior of beta-cyclodextrin dimer with dequalinium chloride (DQC) was studied in an aqueous NH4 Ac-HAc buffer solution of pH 4.00 at room temperature by spectrofluorimetry. The apparent association constant of the complex was 1.67 x 10(4) l mol(-1), which showed higher affinity than native beta-CD (K-s = 4.99 x 10(2) l mol(-1)). Based on the significant enhancement of fluorescence intensity of DQC, a spectrofluorimetric method with high sensitivity and selectivity was developed for the determination of DQC in bulk aqueous solution in presence of beta-CD dimer. The linear range was 6.38-1.60 x 10(3) ng ml(-1) with the detection limit 1.9 ng ml(-1). There was no interference from the excipients normally used in tablets and serum constituents. The proposed method was successfully applied to the determination of DQC in tablets and serum.

• 出版日期2005-12-4
• 单位山东师范大学