A genome-wide 3C-method for characterizing the three-dimensional architectures of genomes

作者：Duan Zhijun^*; Andronescu Mirela; Schutz Kevin; Lee Choli; Shendure Jay; Fields Stanley; Noble William S; Blau C Anthony

来源：Methods, 2012, 58(3): 277-288.

DOI：10.1016/j.ymeth.2012.06.018

• Chromatin
• Chromosome
• Chromosome conformation capture (3C)
• Chromosome conformation capture on chip (4C)
• Genome architecture
• Three-dimensional (3D) organization

Accumulating evidence demonstrates that the three-dimensional (3D) organization of chromosomes within the eukaryotic nucleus reflects and influences genomic activities, including transcription. DNA replication, recombination and DNA repair. In order to uncover structure-function relationships, it is necessary first to understand the principles underlying the folding and the 3D arrangement of chromosomes. Chromosome conformation capture (3C) provides a powerful tool for detecting interactions within and between chromosomes. A high throughput derivative of 3C, chromosome conformation capture on chip (4C), executes a genome-wide interrogation of interaction partners for a given locus. We recently developed a new method, a derivative of 3C and 4C, which, similar to Hi-C, is capable of comprehensively identifying long-range chromosome interactions throughout a genome in an unbiased fashion. Hence, our method can be applied to decipher the 3D architectures of genomes. Here, we provide a detailed protocol for this method. Published by Elsevier Inc.

• 出版日期2012-11