Allele Specific PCR for Genotyping the Probable SNPs in Mitochondrial Genome in Red Jungle Fowl and Domestic Chicken

作者：Ahmed S U; Sharma Deepak^*

来源：Journal of Applied Animal Research, 2010, 38(1): 49-53.

• Allele specific PCR
• red jungle fowl
• SNP
• mitochondrial genome

Allele specific PCR was used for genotyping the identified probable SNP in mitochondrial genome in red jungle fowl and domestic chicken. Out of the total 67 nucleotide substitutions identified on sequence homology comparison of eight complete mitochondrial genome sequences (four each from domestic chicken breeds and red jungle fowl subspecies), 19 were found to be specific for domestic chicken. For six SNPs primers with good score could be designed hence, used for genotyping of the RJF and domestic chicken populations. Allelic frequency of allele specific to domestic fowl and RJF population were 0.94 and 0.89 at COI-114-C/T SNP; 0.83 and 0.89 at COI-255-G/A SNP; 0.78 and 0.94 at COII-279-C/T SNP; 0.94 and 0.83 at ATP6-294-G/A SNP; 0.83 and 0.94 at ND4-969-C/T SNP; and 0.94 and 0.78 at CYTB-543-T/C SNP, respectively. Though the frequency of specific alleles in respective populations was very high, but these alleles were not fixed in the populations hence these SNPs could not be used as population specific markers.

• 出版日期2010-9