Preparative-scale purification of RNA using an efficient method which combines gel electrophoresis and column chromatography

作者：Cunningham L; Kittikamron K; Lu Y

来源：Nucleic Acids Research, 1996, 24(18): 3647-3648.

DOI：10.1093/nar/24.18.3647

Here we describe a reliable method for purifying large amounts of RNA of any sequence and length with comparable efficiency and resolution of gel electrophoresis and with capacity approaching that of column chromatography. The RNA mixture of interest is separated on a cylindrical denaturing polyacrylamide gel, eluted by a peristaltic pump, detected by a UV-vis detector, and collected by a fraction collector, Using this method, we were able to separate one third of a 100 mi in vitro transcribed 34mer hammerhead ribozyme (similar to 6.2 mg) in a single run, The entire 100 mi transcribed RNA (similar to 18.5 mg) was separated after consecutive runs using one single gel preparation.

• 出版日期1996-9-15