D1/D2 Domain of Large-Subunit Ribosomal DNA for Differentiation of Orpinomyces spp

作者：Dagar Sumit S; Kumar Sanjay; Mudgil Priti; Singh Rameshwar; Puniya Anil K^*

来源：Applied and Environmental Microbiology, 2011, 77(18): 6722-6725.

DOI：10.1128/AEM.05441-11

This study presents the suitability of D1/D2 domain of large-subunit (LSU) ribosomal DNA (rDNA) for differentiation of Orpinomyces joyonii and Orpinomyces intercalaris based on PCR-restriction fragment length polymorphism (RFLP). A variation of G/T in O. intercalaris created an additional restriction site for AluI, which was used as an RFLP marker. The results demonstrate adequate heterogeneity in the LSU rDNA for species-level differentiation.

• 出版日期2011-9