A Comparison of microCT and microPET for Evaluating Lymph Node Metastasis in a Rat Model

作者：Flechsig Paul; Kratochwil Clemens; Warth Arne; Rath Daniel; Eichwald Viktoria; Huber Peter E; Kauczor Hans Ulrich; Haberkorn Uwe; Giesel Frederik L^*

来源：Molecular Imaging and Biology, 2016, 18(2): 243-248.

DOI：10.1007/s11307-015-0890-0

• Lung cancer
• Orthotopic lung cancer model
• [F-18]FDG/PET-CT
• Computed tomography

Purpose: The demand to optimize multidisciplinary treatment strategies in patients with benign and malignant diseases of the lung and other organs has led to the increased need of mechanistic proof-of-concept studies in preclinical small animal models using new non-invasive imaging methods. Therefore, we evaluated the role of microPET and microCT for mediastinal lymph node staging in an orthotopic lung cancer model in rats.
Procedures: Human lung cancer cells (NCI-H460) were injected transthoracically in nude rats (NIH-RNU). After 2 weeks of tumour growth, animals underwent multiphase contrast-enhanced microCT using ExiTron nano 12000 as a contrast agent and dynamic microPET using the tracer 2-deoxy-2-[F-18]fluoro-d-glucose ([F-18]FDG). Thereafter, animals were sacrificed for histological analysis.
Results: Late phase micro X-ray computed tomography (microCT) revealed the best delineation of lymph node metastases, as compared to earlier scans. In terms of an increased [F-18]FDG uptake over time, dynamic micro positron emission tomography (microPET) delineated lymph node metastases and enabled metabolic examinations of the induced lung cancer metastases.
Conclusion: The combination of contrast-enhanced microCT and dynamic microPET is feasible in rats for the visualization of mediastinal lymph node metastases.

• 出版日期2016-4