A Sensitive Urinary Lipoarabinomannan Test for Tuberculosis

作者：Hamasur Beston^*; Bruchfeld Judith; van Helden Paul; Kallenius Gunilla; Svenson Stefan

来源：PLos One, 2015, 10(4): e0123457.

DOI：10.1371/journal.pone.0123457

We have previously developed a diagnostic test for tuberculosis based on detection of mycobacterial lipoarabinomannan (LAM) in urine. The method depended on a laborious concentration step. We have now developed an easy to perform test based on a magnetic immunoassay platform, utilizing high avidity monoclonal antibodies for the detection of LAM in urine. With this method the analytical sensitivity of the assay was increased 50-100-fold compared to conventional ELISA. In a pilot study of HIV-negative patients with microbiologically verified TB (n=17) and healthy controls (n=22) the sensitivity of the test was 82% and the specificity 100%. This is in stark positive contrast to a range of studies using available commercial tests with polyclonal anti-LAM Abs where the sensitivity of the tests in HIV-negative TB patients was very low.

• 出版日期2015-4-23
• 单位河北医科大学