A Bacterial Acetyltransferase Destroys Plant Microtubule Networks and Blocks Secretion

作者：Lee Amy Huei Yi^*; Hurley Brenden; Felsensteiner Corinna; Yea Carmen; Ckurshumova Wenzislava; Bartetzko Verena; Wang Pauline W; Van Quach; Lewis Jennifer D; Liu Yulu C; Boernke Frederik; Angers Stephane; Wilde Andrew; Guttman David S; Desveaux Darrell

来源：PLoS Pathogens, 2012, 8(2): e1002523.

DOI：10.1371/journal.ppat.1002523

The eukaryotic cytoskeleton is essential for structural support and intracellular transport, and is therefore a common target of animal pathogens. However, no phytopathogenic effector has yet been demonstrated to specifically target the plant cytoskeleton. Here we show that the Pseudomonas syringae type III secreted effector HopZ1a interacts with tubulin and polymerized microtubules. We demonstrate that HopZ1a is an acetyltransferase activated by the eukaryotic co-factor phytic acid. Activated HopZ1a acetylates itself and tubulin. The conserved autoacetylation site of the YopJ / HopZ superfamily, K289, plays a critical role in both the avirulence and virulence function of HopZ1a. Furthermore, HopZ1a requires its acetyltransferase activity to cause a dramatic decrease in Arabidopsis thaliana microtubule networks, disrupt the plant secretory pathway and suppress cell wall-mediated defense. Together, this study supports the hypothesis that HopZ1a promotes virulence through cytoskeletal and secretory disruption.

• 出版日期2012-2