mu- but not delta- and kappa-opioid receptors in the ventrolateral orbital cortex mediate opioid-induced antiallodynia in a rat neuropathic pain model

作者：Zhao M; Wang JY; Jia H; Tang JS^*

来源：Brain Research, 2006, 1076: 68-77.

DOI：10.1016/j.brainres.2006.01.018

• allodynia
• neuropathic pain
• ventrolateral orbital cortex
• opioid
• opioid receptor
• rat

Previous studies have indicated that the ventrolateral orbital cortex (VLO) is involved in opioid-mediated antinociception in the tail flick test and formalin test. The aim of the current study was to examine the effect of opioids microinjected into the VLO on allodynia in the rat L5/L6 spinal nerve ligation (SNL) model of neuropathic pain and deter-mine the roles of different subtypes of opioid receptors in this effect. The allodynia was assessed by both mechanical (von Frey filaments) and cold plate (4 degrees C) stimuli. Morphine (1.0, 2.5, and 5.0 mu g) microinjected into the VLO contralateral to the nerve ligation dose-dependently depressed the mechanical and cold allodynia,and these effects were reversed by nonselective opioid receptor antagonist naloxone (1.0 mu g) administrated into the same site. Microinjection of endomorphin-1 (5.0 mu g), a highly selective p-opioid receptor agonist, and [D-Ala(2), D-Leu(5)]- enkephalin (DADLE, 10 mu g), a delta-/mu-opioid receptor agonist, also depressed the allodynia, and the effects of both drugs were blocked by selective mu-receptor antagonist-funaltrexamine (beta-FNA, 3.75 mu g), but the effects of DADLE were not influenced by the selective delta-receptor antagonist naltrindole (5.0 mu g). Microinjection of U-62066 (100 mu g), a kappa-opioid receptor agonist, into the VLO had no effect on the allodynia. These results suggest that the VLO is involved in opioid-induced antiallodynia and mu- but not delta- and kappa-opioid receptor mediates these effects in the rat with neuropathic pain.

• 出版日期2006-3-3
• 单位西安交通大学