科研之友
微信
新浪微博
Facebook
分享链接
摘要
Protein tyrosine phosphatases (PTPs) constitute a large family of enzymes that play key roles in Cell signaling Malfunctions of PTP activity have been linked to major human diseases including cancer One key aspect in PTP biology is the elucidation of roles of PTPs, as well as substrates they act on, in different cellular events Herein, a library of 144 putative peptide substrates against different PTPs was synthesized and immobilized onto a glass slide to generate the corresponding phosphopeptide microarray Subsequent screening of the microarray against various PTPs provided a distinctive and comparative substrate fingerprint against each PTP Several new substrates were identified, which might aid in the future design of potent and selective PTPs inhibitors The signal-decrease microarray assay used in our studies provided a facile and efficient way for high-throughput determination of kinetic constants for peptide/PTP in
- 出版日期2010
