Anti-obesity Effect of Komulkosiraegi [Gracilaria vermiculophylla (Ohmi) Papenfuss] Extract in 3T3-L1 Cells

作者：Kim Hyo Min; Kang Seong Il; Shin Hye Sun; Ko Hee Chul; Hong Youn Suk; Kang Seung Woo; Yoon Sun A; Kim Jeong Hwan; Kim Se Jae^*

来源：Food Science and Biotechnology, 2012, 21(1): 83-89.

DOI：10.1007/s10068-012-0010-8

• AMP-activated protein kinase
• anti-adipogenic effect
• Gracilaria vermiculophylla
• 3T3-L1 cell

The ingestion of edible seaweed has long been believed to be beneficial to human health due to its numerous biological actions. In the present study, the ethyl acetate fraction of a komulkosiraegi [Gracilaria vermiculophylla (Ohmi) Papenfuss] ethanol extract (GEFr) was found to potently inhibit adipogenesis of 3T3-L1 preadipocytes, decreasing triglycerol accumulation and the expression of peroxisome proliferator-activated receptor gamma (PPAR gamma), members of the CCAAT/enhancer-binding protein (C/EBP) family, and fatty acid binding protein 2 (aP2). In mature adipocytes, GEFr was found to significantly activate AMP-activated protein kinase (AMPK) by activating liver kinase B1 (LKB1) and stimulating intracellular reactive oxygen species generation. The mRNA levels of genes involved in lipid catabolism were up-regulated. Also, GEFr increased lipolysis in a dose-dependent manner. Taken together, these results suggest that GEFr has potential for use in therapies designed to improve obesity.

• 出版日期2012-2